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MIF Enzymatic Assay from US Patent US11884682: "Compounds and their uses as MIF inhibitors"
Assay data:53 Active, 35 Activity ≤ 1 µM, 86 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Affinity Biochemical interaction (Scintillation proximity assay (SPA; measurement of selective [83H]-BTZO-1 binding to human MIF-His)) EUB0000301b MIF
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Affinity Phenotypic Cellular interaction (Cell viability WST-8 assay (measuring inhibition of apoptosis)) EUB0001135a MIF
Assay data:1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, activity ≤ 1 µMRelated BioAssays by Target
Affinity Phenotypic Cellular interaction (Cell viability WST-8 assay (measuring inhibition of apoptosis)) EUB0000301b MIF
Substrate activity at MIF (unknown origin) assessed as ratio of Kcat to Km
Assay data:4 Tested
SummaryPubMed CitationRelated BioAssays by Target
Substrate activity at MIF (unknown origin) assessed as rate constant Kcat
Substrate activity at MIF (unknown origin) assessed as rate constant Km
Non-competitive inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate preincubated for 1 hr followed by substrate addition by Lineweaver-Burk plot anlysis
Assay data:5 Active, 5 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Competitive inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate preincubated for 1 hr followed by substrate addition by Lineweaver-Burk plot anlysis
Assay data:1 Active, 1 Tested
Inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate preincubated for 1 hr followed by substrate addition by Lineweaver-Burk plot anlysis
Assay data:6 Tested
Reversal inhibition of MIF tautomerase (unknown origin) at 0.5 mM using 4-HPP as substrate preincubated for 30 min followed by 100 fold dilution by dilution assay
Assay data:6 Active, 6 Tested
Inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate preincubated for 1 hr followed by substrate addition
Assay data:10 Tested
Inhibition of inhibition of MIF tautomerase (unknown origin) in MIF-induced mouse RAW264.7 cells assessed as reduction in cell migration relative to control
Assay data:7 Tested
Potency index ratio of ISO-1 IC50 to test compound IC50 for inhibition of MIF tautomerase (unknown origin) using 4-HPP as substrate
Assay data:3 Tested
LYTAC activity at ASGPR/MIF (unknown origin) by cell based assay
SummaryCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant human MIF tautomerase activity using 4-hydroxyphenyl pyruvic acid (HPP) as substrate pre-incubated for 30 mins and followed by susbtrate addition and
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition Assay from US Patent US10968198: "Biaryltriazole inhibitors of macrophage migration inhibitory factor"
Assay data:31 Active, 20 Activity ≤ 1 µM, 35 Tested
Enzyme Assay from US Patent US8618147: "Modified macrophage migration inhibitory factor inhibitors"
Assay data:8 Active, 16 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Tautomerase Enzyme Assay from Article 10.1074/jbc.M110.113951: "Identification and characterization of novel classes of macrophage migration inhibitory factor (MIF) inhibitors with distinct mechanisms of action."
Assay data:17 Active, 8 Activity ≤ 1 µM, 18 Tested
Tautomerase Activity Assay from Article 10.1074/jbc.M112.341321: "Novel Anti-inflammatory Activity of Epoxyazadiradione against Macrophage Migration Inhibitory Factor: INHIBITION OF TAUTOMERASE AND PROINFLAMMATORY ACTIVITIES OF MACROPHAGE MIGRATION INHIBITORY FACTOR."
Assay data:1 Active, 4 Tested
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