|
| Status |
Public on Dec 31, 2022 |
| Title |
12-215-LN |
| Sample type |
genomic |
| |
|
| Source name |
12-215-LN
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 12-215-LN
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was quantified by Quant-iT PicoGreen dsDNA Reagent (Invitrogen) and the integrity was analyzed in a 1.3% agarose gel. Bisulfite conversion of 600 ng of each sample was perform according to the manufacturer's recommendation for Illumina Infinium Assay. Effective bisulphite conversion was checked for three controls that were converted simultaneously with the samples. 4 ul of bisulfite converted DNA were used to hybridize on Infinium HumanMethylation 450 BeadChip, following Illumina Infinium HD Methylation protocol. Chip analysis was performed using Illumina HiScan SQ fluorescent scanner.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using High Scan SQ using standard recommended Illumina scanner setting
|
| Description |
Melanoma patient-derived short term culture
|
| Data processing |
Genome Studio V2011.2
|
| |
|
| Submission date |
Sep 14, 2022 |
| Last update date |
Dec 31, 2022 |
| Contact name |
IJC Bioinformatics |
| E-mail(s) |
ijcbioinfo@carrerasresearch.org
|
| Organization name |
Josep Carreras Leukemia Research Institute
|
| Lab |
Bioinformatics Unit
|
| Street address |
Josep Carreras Building, Ctra de Can Ruti, Camà de les Escoles, s/n
|
| City |
Badalona |
| State/province |
Catalonia |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE213382 |
An epigenetic switch controls the expression of an alternative NR2F2 isoform that unleashes a pro-metastatic program in melanoma [450K] |
| GSE213392 |
An epigenetic switch controls the expression of an alternative NR2F2 isoform that unleashes a pro-metastatic program in melanoma. |
|
