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Items: 4

1.

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts [protein occupancy profiling]

(Submitter supplied) Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation and translation. We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteomics and to display the protein occupancy on mRNA transcripts by next-generation sequencing. Application to a human embryonic kidney cell line identified close to 800 proteins. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
2 Samples
Download data: BEDGRAPH
Series
Accession:
GSE38355
ID:
200038355
2.

Illumina HiSeq 2000 (Homo sapiens)

Platform
Accession:
GPL11154
ID:
100011154
3.

profiling library 2

Organism:
Homo sapiens
Source name:
HEK293 cell culture
Platform:
GPL11154
Series:
GSE38157 GSE38355
Download data
Sample
Accession:
GSM940576
ID:
300940576
4.

profiling library 1

Organism:
Homo sapiens
Source name:
HEK293 cell culture
Platform:
GPL11154
Series:
GSE38157 GSE38355
Download data
Sample
Accession:
GSM940575
ID:
300940575
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Supplemental Content

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