GEO DataSets

(Submitter supplied) We conducted a genome-wide survey of genes in Ae. aegypti females that are transcriptionally responsive upon challenge with dengue virus (serotype-2). The array was designed with 60-mer oligos specific to 16,092 gene transcripts of gene build AaegL1.1 (www. vectorbase.org). The hybridizations were performed at NimbleGen. We provided total RNA purified from the infected and control samples to NimbleGen. more...

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL8705

15 Samples

Download data: PAIR

(Submitter supplied) We have developed genetically modified Ae. aegypti mosquitoes that activate the conserved antiviral JAK/STAT pathway in the fat body tissue, by overexpressing either the receptor Dome or the Janus kinase Hop by the blood feeding-induced vitellogenin (Vg) promoter. Transgene expression inhibits infection with several dengue virus (DENV) serotypes in the midgut as well as systemically and in the salivary glands. more...

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL20714

16 Samples

Download data: TXT

(Submitter supplied) The dengue virus (DENV) cause frequent epidemics infecting ~390 million people annually in over 100 countries. There are no approved vaccines or antiviral drugs for treatment of infected patients. However, there is a novel approach to control transmission of DENV by the mosquito vectors, Aedes aegypti and Ae. albopictus, using Wolbachia symbiont. The wMelPop strain of Wolbachia suppresses DENV transmission and shortens the mosquito life span. more...

Organism:

Aedes albopictus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26091

11 Samples

Download data: TXT

(Submitter supplied) Dengue viruses (DENV) are generally maintained in a cycle which requires horizontal transmission via their arthropod vector, Ae. Aegypti, to the vertebrate host. One important consequence of this process is the interference of these arboviruses with both invertebrate and vertebrate immune systems. While infection of vertebrates causes disease, the presence of DENV gives rise to life-long, persistent infection in mosquitoes. more...

Organism:

Aedes aegypti

Type:

Expression profiling by SAGE

Platform:

GPL10360

2 Samples

Download data

(Submitter supplied) Microarray analysis on days 1, 2 and 7 post-infection with dengue, yellow fever and West Nile virus in Aedes aegypti Rockefeller strain mosquitoes RNA was purified and hybridized with Nimblegen X4 microarray chips using 81-mer probes designed from 18,000 open reading frames (ORF) found in the Ae. aegypti genome, with 2 different probes per ORF

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL13335

37 Samples

Download data: PAIR, TXT

(Submitter supplied) Analysis of Culex quinquefasciatus responses to West Nile virus (WNV) infection at 7 and 14 days after ingestion of infected blood in the gut and carcass tissues.

Organism:

Culex quinquefasciatus

Type:

Expression profiling by array

Platform:

GPL10712

16 Samples

Download data

(Submitter supplied) RNA-Seq was used for transcriptome sequencing of 0-12 hour embryos.

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11087

4 Samples

Download data: BED

(Submitter supplied) Mosquitoes transmit many flaviviruses of global public health significance. Efficient viral transmission to mammalian hosts requires mosquito salivary factors that modulate local host responses, such as recruitment of virus-permissive myeloid cells to the bite sites. However, the specific salivary components facilitating viral transmission and their mechanisms of action remain largely unknown. Here, we showed that a female mosquito salivary gland-specific protein, named Aedes aegypti Neutrophil Recruitment Protein (AaNRP), acts as a key salivary component to facilitate the transmission of Zika (ZIKV) and dengue (DENV) viruses. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: MTX, TSV

(Submitter supplied) Background. Aedes aegypti is arguably the most studied of all mosquito species in the laboratory and is the primary vector of both Dengue and Yellow Fever flaviviruses in the field. A large number of transcriptional studies have been made in the species and these usually report transcript quantities observed at a certain age or stage of development. However, circadian oscillation is an important characteristic of gene expression in many animals and plants, modulating both their physiology and behavior. more...

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL4877

12 Samples

Download data: GPR, TXT

(Submitter supplied) In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 .

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

8 Samples

Download data: CEL, CHP

(Submitter supplied) In order to analyze the global changes in gene expression resulting from loss of Fra signaling, we performed a microarray experiment comparing Drosophila embryos containing a loss of function fra[3] mutation to age matched wildtype embryos. RNA extracted from fra[3] mutant vs. wildtype embryos were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 .

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL1322

8 Samples

Download data: CEL, CHP

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify transcripts that are specific or enriched in circulating hemocytes compared to either neuronal or to the rest of the body.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

16 Samples

Download data: CEL, CHP

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify if and to what degree circulating hemocytes have altered global expression profiles after infection with the rodent malaria parasite, Plasmodium berghei

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

6 Samples

Download data: CEL, CHP

(Submitter supplied) Strain variation in Aedes aegypti transcriptome

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11087

8 Samples

Download data: BAM

(Submitter supplied) We present a microarray study of hemocyte-enriched transcriptome in Aedes aegypti. We directed our efforts to critically assess the transcriptomic features distinctive of hemocytes, and discussed our findings in relation to infection-responsive genes and immune-relate gene families. Specifically, our analysis examined how tissue-enriched expression patterns change with the immune status of the host, and resolved patterns of transcriptional change unique to hemocytes from those that are likely shared by other immune responsive tissues.

Organism:

Aedes aegypti

Type:

Expression profiling by array

Platform:

GPL4877

40 Samples

Download data: GPR

(Submitter supplied) Parasite biology, by its very nature, cannot be understood without integrating it with that of the host, nor can the host response be adequately explained without considering the activity of the parasite. However, due to experimental limitations, molecular studies of parasite-host systems have been predominantly one-sided investigations focusing on either of the partners. Here we conduct a joint dual RNA-seq time course analysis of filarial parasite and host mosquito to better understand the parasite processes underlying development in, and interaction with, the host tissue from the establishment of infection to the emergence of infective-stage larva. more...

Organism:

Aedes aegypti

Type:

Expression profiling by high throughput sequencing

Platform:

GPL14869

38 Samples

Download data: TXT

(Submitter supplied) Embryos generated with the use of assisted reproductive technologies (ART) can develop overgrowth syndromes. In ruminants, the condition is referred to as large offspring syndrome (LOS) and exhibits variable phenotypic abnormalities including overgrowth, enlarged tongue, and abdominal wall defects. These characteristics recapitulate those observed in the human loss-of-imprinting (LOI) overgrowth syndrome Beckwith-Wiedemann (BWS). more...

Organism:

Bos indicus x Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21383

32 Samples

Download data: TXT

(Submitter supplied) The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective fashion. Here, we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67X coverage, Sample GSM1551550). We then combine our method with draft sequences to create genome assemblies of the mosquito disease vectors Aedes aegypti and Culex quinquefasciatus, each consisting of three scaffolds corresponding to the three chromosomes in each species. more...

Organism:

Aedes aegypti; Culex quinquefasciatus; Homo sapiens

Type:

Other; Third-party reanalysis

Platforms:

GPL22030 GPL22042

3 Samples

Download data: FASTA, TXT

(Submitter supplied) We analyze whether tRNA quantifications based on small RNA-seq data are informative enough to distinguish between different human cell lines covering multiple tissue types. We therefore apply both small RNA-seq and Hydro-tRNAseq to HEK293 (kidney), HCT116 (colon), HeLa (cervix), MDA-MB-231 (breast), and BJ fibroblasts. First, the correlations between the two methods of identical samples and computational mapping pipeline range between 0.93 and 0.96 for all cell lines. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16791

30 Samples

Download data: CSV