GEO DataSets

(Submitter supplied) Purpose: For patients with osteosarcoma, disease-related mortality most often results from lung metastasis—a phenomenon shared with many solid tumors. While established metastatic lesions behave aggressively, very few of the tumor cells that reach the lung will survive. By identifying mechanisms that facilitate survival of disseminated tumor cells, we can develop therapeutic strategies that prevent and treat metastasis. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL25431 GPL25526

5 Samples

Download data: TAR

(Submitter supplied) To investigate the molecular and cellular alterations occurring in the lung tissue during the pre-metastatic niche formation in osteosarcoma, we used two different approaches in which mice were implanted subcutaneously with the 143B OS cells for a primary tumour (PT) formation or treated with the 143B cell-derived secretome (SCR) to mimic the release of secreted factors by a locally primary tumour.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: XLSX

(Submitter supplied) Osteosarcoma cells were grown in cell culture, as subcutaneous flank tumors, orthotpic primary tumors or experimental metastases to study the role of intra-tumor transcriptional heterogeneity on adaptations in tumor cells that faciliate tissue colonization.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL20301 GPL24247

22 Samples

Download data: TAR

(Submitter supplied) SaOS2 osteosarcoma cells were cultured with or without atorvastatin (10 µM) for 6, 15 or 24h (2 biological replcates). RNA were isolated and hybridized to RNG microarrays. Background Osteosarcoma is the most common primary tumor of bone. The rapid development of metastatic lesions and resistance to chemotherapy remain major mechanisms responsible for the failure of treatments and poor survival rate for patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1456

6 Samples

Download data: GPR

(Submitter supplied) Metastasis results from a complex set of traits acquired by tumor cells, distinct from those necessary for tumorigenesis. Here, we investigate the contribution of enhancer elements to the metastatic phenotype of osteosarcoma. Through epigenomic profiling, we identify substantial differences in signature enhancer-histone marks between near-isogenic pairs of high and low lung-metastatic osteosarcoma cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL11154 GPL16791

91 Samples

Download data: BED, BW, TXT

(Submitter supplied) To identify OS-specific gene alterations, 38 tumor samples were collected from 29 unique patients with osteosarcoma. We performed RNA-sequencing on 28 primary osteosarcoma tumors and 10 metastatic osteosarcoma tumors. We compared the primary and metastatic genomic signatures of all 38 samples to discover differentially expressed genes.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

38 Samples

Download data: TXT

(Submitter supplied) To investigate the functions of the RNA-binding proteins LARP4A and LARP4B in prostate cancer and osteosarcoma, we knocked down LARP4A and LARP4B in PC3, MG63 and MNNG/HOS cell lines and characterised the effects on in vivo tumourigenicity, lung colonisation and in vitro proliferation, cell cycle progression, apoptosis and migration. In order to uncover potential mechanisms behind the phenotypes that we found, we then performed transcriptomic profiling using bulk RNAseq and used the data to identify potential biological processes influenced by LARP4A/LARP4B loss

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

12 Samples

Download data: TXT