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Platform GPL32887 Query DataSets for GPL32887
Status Public on Nov 08, 2023
Title LAM Antigens Array
Technology type spotted peptide or protein
Distribution non-commercial
Organism synthetic construct
Manufacturer Todd Lowary, University of Alberta and Engineering Arts Tempe Arizona
Manufacture protocol Microarray slides were printed at Engineering Arts LLC (Phoenix, AZ). BSA conjugates were noncontact printed on Schott type E slides (Schott North America, Inc., Elmsford, NY) using an Engineering Arts au301 Rainmaker microarray printer and dispensed at 100 ?g/mL in buffer (1:10 phosphate-buffered saline plus 0.005% vol/vol Triton X-100). Antigens were dispensed at 360 pL per spot to produce 140- to 160-?m diameter spots in triplicate. Relative humidity was maintained at 65%; slides were subjected to white light for 100% inline drop inspection. Printing was carried out in a HEPA-filtered environment at room temperature. Printed slides were stored at ?20 °C until use. Each slide contains 24 identical array containing 30 components printed in duplicate.
 
Description Synthetic antigens coupled to bovine serum albumin, Subarray 30 and Subarray 61
 
Submission date Nov 28, 2022
Last update date Nov 08, 2023
Contact name Jacqueline M. Achkar
Organization name Albert Einstein College of Medicine
Street address 1300 Morris Park Avenue
City Bronx
ZIP/Postal code 10461
Country USA
 
Samples (2) GSM6758069, GSM6758091
Series (1)
GSE218873 Features and protective efficacy of human monoclonal antibodies targeting M. tuberculosis arabinomannan

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