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Series GSE100865 Query DataSets for GSE100865
Status Public on Dec 05, 2017
Title Differential ability of pandemic and seasonal H1N1 influenza 1 A virus to alter function of human neutrophils
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Neutrophils are essential cells of host innate immunity. Although the role of neutrophils in defense against bacterial and fungal infections is well characterized, there is relative paucity of information about their role against viral infections. Influenza A virus (IAV) infection can be associated with secondary bacterial co-infection, and it has long been posited that the ability of IAV to alter normal neutrophil function predisposes individuals to secondary bacterial infections. To better understand this phenomenon, we evaluated the interaction of pandemic or seasonal H1N1 IAV with human neutrophils isolated from healthy subjects. These viruses were ingested by human neutrophils and elicited changes in neutrophil gene expression that are consistent with an interferon-mediated immune response. Viability of neutrophils following co-culture with either pandemic and seasonal H1N1 IAV was similar for up to 18 h of culture. Notably, neutrophil exposure to seasonal IAV (but not pandemic IAV) primed these leukocytes for enhanced functions, including production of reactive oxygen species and bactericidal activity. Taken together, our results are at variance with the universal idea that IAV impairs neutrophil function directly to predispose individuals to secondary bacterial infections. Rather, we suggest some strains of IAV prime neutrophils for enhanced bacterial clearance.
 
Overall design time course with three treatments, Kawasaki, Mex09 and RPMI
 
Contributor(s) Malachowa N, Freedman B, Sturdevant DE, Kobayashi SD, Nair V, Feldmann F, Starr T, Steele-Mortimer O, Kash JC, Taubenberger JK, Feldmann H, DeLeo FR
Citation(s) 29299535
Submission date Jul 06, 2017
Last update date Jul 25, 2021
Contact name Dan Sturdevant
E-mail(s) dsturdevant@niaid.nih.gov
Phone 4063639248
Organization name NIH
Department NIAID
Lab RTS
Street address 903 S 4th street
City Hamilton
State/province MT
ZIP/Postal code 59840
Country USA
 
Platforms (1)
GPL16686 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
Samples (35)
GSM2695344 Neutrophils from Donor 6 infected MOI 5 with IVA strain Kawasaki time point 1 hour
GSM2695345 Neutrophils from Donor 8 infected MOI 5 with IVA strain Kawasaki time point 1 hour
GSM2695346 Neutrophils from Donor 9 infected MOI 5 with IVA strain Kawasaki time point 1 hour
Relations
BioProject PRJNA393311

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100865_RAW.tar 292.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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