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| Status |
Public on Dec 31, 2017 |
| Title |
Alterations of microRNAs throughout the malignant evolution of cutaneous squamous cell carcinoma: The role of miR-497 in epithelial to mesenchymal transition of keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We found that miR-497 expression decrease in cSCC biopsies and cell lines compare to normal skin biopsies or normal keratinocytes cell. To understand in role lost expression of miR-497 might have in tumorigenesis of keratinocytes, we aimed to fine possible biochemical pathways and possible targets of miR-497 in cSCC. In order do so, we over expressed miR-497 in cSCC and used microarrays to fine global gene expression effected by miR-497.
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| Overall design |
Two cSCC cell lines Met-1 and SCL-II were generated, each were either over-expressing miR-497 or control plasmid. From these cells, RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Mizrahi A, Barzilai A, Gur-Wahnon D, Ben-Dov IZ, Glassberg S, Meningher T, Elharar E, Masalha M, Jacob-Hirsch J, Tabibian-Keissar H, Barshack I, Roszik J, Leibowitz-Amit R, Sidi Y, Avni D |
| Citation(s) |
28925390 |
| Submission date |
Jul 10, 2017 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
j-jacob@sheba.health.gov.il
|
| Phone |
0523790500
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| Organization name |
Sheba Medical Center at Tel HaShomer
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| Street address |
haChevel 33
|
| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA393708 |
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