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| Status |
Public on Jul 12, 2017 |
| Title |
Expression data from HUVECs with ADK shRNA knockdown |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The molecular mechanisms underlying vascular inflammation and associated inflammatory vascular diseases are not well defined. Here we show that endothelial intracellular adenosine and its key regulator adenosine kinase (ADK) play important roles in vascular inflammation. Pro-inflammatory stimuli lead to endothelial inflammation by increasing endothelial ADK expression, reducing the level of intracellular adenosine in endothelial cells, and activating the transmethylation pathway through increasing the association of ADK with S-adenosylhomocysteine (SAH) hydrolase (SAHH). Increasing intracellular adenosine by genetic ADK knockdown or exogenous adenosine reduces activation of the transmethylation pathway and attenuates the endothelial inflammatory response. In addition, loss of endothelial ADK in mice leads to reduced atherosclerosis and affords protection against ischemia/reperfusion injury of the cerebral cortex. Taken together, these results demonstrate that intracellular adenosine, which is controlled by the key molecular regulator ADK, influences endothelial inflammation and vascular inflammatory diseases.
We knocked down the adenosine kinase (ADK) in human primary endothelial cells to study the endothelial inflammatory responses to ADK inactivation under static conditions.
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| Overall design |
HUVECs were infected with human ADK shRNA or control adenovirus and RNA was processed for microarray analysis 30 h post infection. Three biological replicates were used for each condition.
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| Contributor(s) |
Xu Y, Huo Y |
| Citation(s) |
29038540 |
| Submission date |
Jul 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Yiming Xu |
| E-mail(s) |
yixu@augusta.edu
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| Phone |
706-721-4417
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| Organization name |
Augusta University
|
| Department |
Vascular Biology Center
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| Lab |
Huo lab
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| Street address |
1459 Laney Walker Blvd
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| City |
Augusta |
| State/province |
Georgia |
| ZIP/Postal code |
30912 |
| Country |
USA |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA393776 |
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