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| Status |
Public on Aug 31, 2017 |
| Title |
Genomic binding profiling upon expression of Ubx in S2 cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We sought to determine the genomic binding profile of the Drosophila Hox protein Ubx in a homogenous cell system. S2-DRSC cells that have no Hox expression were stably transfected with HA-tagged Ubx under the control of a metallothionein promoter. Upon Ubx expression, we identified high enrichment of Ubx at a large number transcription start sites that colocalises with the GAGA and M1BP.
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| Overall design |
S2-DRSC cells are stably transfected with HA-tagged Ubx (S2-DRSC:Ubx).S2-Ubx cells are analysed for genomic binding of HA-Ubx
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| Contributor(s) |
Zouaz A, Auradkar A, Delfini MC, Macchi M, Barthez M, Akoa SE, Bastianelli L, Levine SS, Graba Y, Saurin AJ |
| Citation(s) |
28871058, 32541927 |
| Submission date |
Jul 18, 2017 |
| Last update date |
Jun 22, 2020 |
| Contact name |
Andrew J Saurin |
| E-mail(s) |
andrew.saurin@univ-amu.fr
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| Organization name |
IBDM / CNRS
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| Street address |
Campus de Luminy
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| City |
Marseille |
| ZIP/Postal code |
13009 |
| Country |
France |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE101557 |
Expression of Hox transcription factors in Drosophila S2 cells |
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| Relations |
| BioProject |
PRJNA394835 |
| SRA |
SRP112701 |
