 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 19, 2019 |
| Title |
Evolution of an X-linked miRNA family predominately expressed in mammalian male germ cells |
| Organisms |
Sus scrofa; Oryctolagus cuniculus; Mus musculus; Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
microRNAs (miRNAs) are important post-transcriptional regulators of gene expression. However, comprehensive expression profiles of miRNAs during spermatogenesis in mammals is lacking. In the present study, we sequenced small RNAs in highly purified spermatogonial stem cells (SSCs), spermatocytes in the pre-leptotene, leptotene, zygotene and pachytene stages, haploid round spermatids and spermatozoa. We found that a family of X-linked miRNAs, named spermiRs, is predominantly expressed in spermatogonia and early meiotic phases. Profiling of small RNAs in different mammalian tissues revealed a conserved high expression pattern of spermiRs in testes. spermiR appears to originate from THER1 in marsupial, a retrotransposon that is extinct in current mammals. It has expanded rapidly and has diversified into two clusters, spermiR-L and spermiR-R, by tandem duplication, which was partially mediated by flanking retrotransposable elements. Notably, despite the highly frequent lineage-specific duplication events, all spermiR family members are strictly located between two conserved protein coding genes, Slitrk2 and Fmr1. Intriguingly, spermiR-Ls and spermiR-Rs have evolved different expression preferences during spermatogenesis in different mammals. Moreover, the sequences of spermiRs and their precursors, including the seed regions, are highly divergent within and among mammals. However, their target genes are largely overlapping in male reproduction-related pathways. When miR-741, the most highly expressed spermiRs during mouse spermatogenesis, was knocked out in SSCs, another spermiR, miR-465, was dramatically up-regulated and became the most abundant one, indicating functional compensation between spermiR family members. All these observations indicate a strong co-evolution between spermiRs and their targets.
|
| |
|
| Overall design |
Small RNA profiling of purified male germ cells during mouse spermatogenesis, different stages of rabbit testis, adult pig and rat testis, and miR-741 knock-out SSCs by TALEN and CRISPR.
|
| |
|
| Contributor(s) |
Zhang F, Zhang Y, Wu L |
| Citation(s) |
30649414 |
| Submission date |
Jul 26, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Fengjuan Zhang |
| Organization name |
Shanghai Institutes for Biological Sciences, CAS
|
| Department |
Institute of Biochemistry and Cell Biology
|
| Lab |
Ligang Wu
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platforms (4)
|
| GPL11429 |
Illumina HiSeq 2000 (Sus scrofa) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
| GPL18453 |
Illumina HiSeq 2000 (Oryctolagus cuniculus) |
|
| Samples (20)
|
| GSM2719092 |
Mouse wild type cultured spermatogonia stem cells |
| GSM2719093 |
Mouse wild type pre-leptotene spermatocytes |
| GSM2719094 |
Mouse wild type leptotene spermatocytes |
| GSM2719095 |
Mouse wild type zygotene spermatocytes |
| GSM2719096 |
Mouse wild type pachytene spermatocytes |
| GSM2719097 |
Mouse wild type round sperms |
| GSM2719098 |
Mouse wild type mature sperms |
| GSM2719099 |
Rabbit wild type testes postnatal day 10 |
| GSM2719100 |
Rabbit wild type testes postnatal day 30 |
| GSM2719101 |
Rabbit wild type testes postnatal day 60 |
| GSM2719102 |
Rabbit wild type testes postnatal day 70 |
| GSM2719103 |
Rabbit wild type testes postnatal day 80 |
| GSM2719104 |
Rabbit wild type testes postnatal day 90 |
| GSM2719105 |
Rabbit wild type testes postnatal day 110 |
| GSM2719106 |
Rabbit wild type testes postnatal day 180 |
| GSM2719107 |
Pig wild type adult testes |
| GSM2719108 |
Rat wild type adult testes |
| GSM2719109 |
Mouse wild type cultured spermatogonia stem cells control |
| GSM2719110 |
Mouse miR-741 knock-out by TALEN cultured spermatogonia stem cells |
| GSM2719111 |
Mouse miR-741 knock-out by CRISPR cultured spermatogonia stem cells |
|
| Relations |
| BioProject |
PRJNA395954 |
| SRA |
SRP113652 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE101933_RAW.tar |
229.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...