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| Status |
Public on Oct 04, 2017 |
| Title |
Alignment of Mitotic Chromosomes in Human Cells Involves SR-Like Splicing Factors Btf and TRAP150 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Here we show that depletion of the homologous non-classical serine-arginine-rich (SR) splicing factors Btf (BCLAF1) and TRAP150 causes mitotic defects.
We propose that, in addition to their previously reported roles in maintaining mRNA distribution, Btf and TRAP150 control abundance of transcripts encoding mitotic regulators thereby affecting mitotic progression in human cells.
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| Overall design |
Affymetrix Exon 1.0 ST microarrays were performed according to the manufacturer's directions with mRNA isolated from cultured HeLa cells treated with siRNA targeting Btf, TRAP150, or a control siRNA.
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| Contributor(s) |
Michael P. M, Paula B |
| Citation(s) |
28895891 |
| Submission date |
Aug 09, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Michael Markey |
| E-mail(s) |
michael.markey@wright.edu
|
| Phone |
937-775-4536
|
| Fax |
937-775-3031
|
| URL |
http://www.med.wright.edu/cgr/
|
| Organization name |
Wright State Unviersity
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Center for Genomics Research
|
| Street address |
3640 Colonel Glenn Hwy.
|
| City |
Dayton |
| State/province |
OH |
| ZIP/Postal code |
45435 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA397779 |
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