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| Status |
Public on Oct 31, 2017 |
| Title |
RNA sequencing of macrophages derived from monocytes differentiated in presence of M-CSF, GM-CSF and heat killed mycobacteria M. obuense |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Earlier work has shown that macrophages derived from differentiation of monocytes by heat killed (HK) mycobacteria (e.g. M. obuense) exhibit unique immunophenotypic and molecular properties. Yet, our knowledge of these properties is still limited. The goal of the study is to understand global gene expression programs that are differentially modulated in macrophages derived from monocytes that were differentiated through three different routes: in presence of M-CSF, GM-CSF and the heat killed mycobacterium M. obuense. We performed RNA sequencing (RNA-Seq) of monocyte-derived macrophages (MDM) that were acquired from four separate healthy donors and differentiated by incubation with M-CSF (M-MDM), GM-CSF (GM-MDM) and HK M. obuense (Mob-MDM) (n = 12 samples). We report that Mob-MDM exhibit unique gene expression programs that may explain its unique immunophenotypic properties and thus immunomodulatory capacity.
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| Overall design |
We performed RNA-Seq of monocyte-derived macrophages (MDM) that were acquired from four phenotypically healthy donors. Monocytes were differentiated by incubation with M-CSF (M-MDM), GM-CSF (GM-MDM) and HK M. obuense (Mob-MDM) (n = 12 samples).
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| Contributor(s) |
Bazzi S, Kadara H, Bahr G |
| Citation(s) |
29046677 |
| Submission date |
Aug 10, 2017 |
| Last update date |
Jul 09, 2021 |
| Contact name |
Humam Kadara |
| Organization name |
University of Texas MD Anderson Cancer Center
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| Street address |
2130 W. Holcombe Blvd
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA397874 |
| SRA |
SRP115201 |
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