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Status |
Public on Jul 11, 2018 |
Title |
Determination of direct binding targets of Quaking RNA binding proteins |
Organism |
Mus musculus |
Experiment type |
Other
|
Summary |
Identication of RNA molecules directly bound by Quaking proteins in mouse myoblasts and myotubes
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Overall design |
Individual nucleotide resolution crosslinking immunoprecipitation of Qk proteins in either C2C12 myoblasts or 72h differentiated myotubes; control libraries were constructed using mouse anti-HA antibody from C2C12 myoblast cross-linked lysate, other libraries used either mouse anti-PanQk, mouse anti-Qk6, or mouse anti-Qk7 antibody
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Contributor(s) |
Fagg WS, Shiue L, Katzman S, Ares M |
Citation(s) |
29021242, 31437148 |
Submission date |
Aug 14, 2017 |
Last update date |
Sep 18, 2019 |
Contact name |
W. Samuel Fagg |
E-mail(s) |
sam.fagg@gmail.com
|
Organization name |
University of Texas Medical Branch
|
Department |
Surgery/Biochemistry and Molecular Biology
|
Street address |
301 University Blvd, Department of Surgery, MRB5.150, L21875
|
City |
Galveston |
State/province |
TX |
ZIP/Postal code |
77551 |
Country |
USA |
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Platforms (1) |
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Samples (6)
|
GSM2741756 |
anti-PanQk iCLIP, 72h differentiated C2C12 myotubes (MiSeq pilot) |
GSM2741757 |
anti-Qk7 iCLIP, C2C12 myoblasts (MiSeq pilot) |
GSM2741758 |
anti-HA iCLIP, C2C12 myoblasts (MiSeq + HiSeq) |
GSM2741759 |
anti-PanQk iCLIP, C2C12 myoblasts (MiSeq + HiSeq) |
GSM2741760 |
anti-PanQk iCLIP 72h differentiated C2C12 myotubes (MiSeq + HiSeq) |
GSM2741761 |
anti-Qk6 iCLIP, C2C12 myoblasts (MiSeq) |
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This SubSeries is part of SuperSeries: |
GSE102615 |
Quaking isoform depletion in C2C12 myoblasts; direct binding targets of Quaking RNA binding proteins |
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Relations |
BioProject |
PRJNA398228 |
SRA |
SRP115394 |