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| Status |
Public on Aug 01, 2022 |
| Title |
Epigenetic and transcriptomic signature reveals Sox8 as an otic master regulator |
| Organism |
Gallus gallus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Previously FGF signalling has been shown to specify otic fate from multipotent pre-placodal ectoderm. Though many FGF target genes have been identified in subsequent studies, none of them is sufficient to induce otic lineage. Profiling and analysing the transcriptome and epigenomical signature during otic specification, we predicted and confirmed Sox8 as an otic master regulator, able to specify otic fate in the pre-placodal ectoderm. This is achieved by activating the expression of otic identity markers and compromising the fate towards lens and trigeminal ganglion. In the combat to differentiate ESCs/iPSCs or convert other cell lineage into otic lineage, our discovery may add another weaponry to tame these cells towards otic cells.
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| Overall design |
ChIPseq of H3K27ac, H3K4me3, H3K27me3, and ATACseq were performed with a single biological replicate. Input was included for the ChIPseq experiments.
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| |
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| Contributor(s) |
Chen J, Thiery A, Streit A |
| Citation(s) |
35867760 |
| Submission date |
Aug 18, 2017 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Andrea Streit |
| E-mail(s) |
andrea.streit@kcl.ac.uk
|
| Organization name |
Kings College London
|
| Department |
Centre for Craniofacial & Regenerative Biology
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| Lab |
Streit
|
| Street address |
Centre for Craniofacial & Regenerative Biology, King's College London
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16133 |
Illumina HiSeq 2000 (Gallus gallus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA399076 |
| SRA |
SRP115832 |
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