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Status |
Public on Jul 12, 2018 |
Title |
Whole genome bisulphite sequencing using the Illumina X system [Bisulfite-Seq] |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
While Illumina X technology has helped to reduce the cost of whole genome sequencing substantially, its application for bisulphite sequencing is not straightforward. We describe the optimization of a library preparation and sequencing approach that maximizes the yield and quality of sequencing, and how to eliminate a previously unrecognized artefact affecting several percent of bisulphite sequencing reads.
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Overall design |
Whole genome bisulphite sequencing of NA12878 and IMR90 cell lines using BS-tagging protocol
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Contributor(s) |
Suzuki M, Liao W, Wos F, Johnston AD, DeGrazia J, Ishii J, Bloom T, Zody MC, Germer SM, Greally JM |
Citation(s) |
30093547 |
Submission date |
Sep 05, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Andrew Johnston |
E-mail(s) |
Andrew.Johnston@med.einstein.yu.edu
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Organization name |
Albert Einstein College of Medicine
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Department |
Genetics
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Lab |
Price 314
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Street address |
1301 Morris Park Avenue
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City |
Bronx |
State/province |
NY |
ZIP/Postal code |
10461 |
Country |
USA |
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Platforms (1) |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
GSE103505 |
Whole genome bisulphite sequencing using the Illumina X system |
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Relations |
BioProject |
PRJNA401763 |
SRA |
SRP116948 |