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| Status |
Public on Jun 09, 2020 |
| Title |
Expression data from Control or TRIB3-silenced A549 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To determine the critical mediator of TRIB3-enhanced EGFR recycling, we examined the expression profile of genes that might regulate EGFR recycling by using mRNA microarrays.
EGFR tyrosine kinase inhibitors (TKIs) confer first line therapy for patients with non-small-cell lung cancer (NSCLC). However, patients eventually develop disease progression, often driven by inevitable acquisition of EGFR TKI resistant mutations. Currently all EGFR TKIs repress NSCLC through inhibiting the kinase activity of EGFR signaling, highlighting the need for therapeutics with alternative mechanisms of action. Here we report that the elevated TRIB3 expression associates positively with EGFR stability and NSCLC progression. TRIB3 interacts with EGFR and recruits PKCĪ± thereby enhances PKCĪ±-induced T654 phosphorylation, which suppresses EGFR degradation and enhances membrane recycling, EGFR downstream signaling, and NSCLC stemness.
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| Overall design |
Total RNA from A549 cells expressing Control-shRNA or TRIB3-shRNA were obtained. RNA quantity and quality were measured by NanoDrop ND-1000. RNA microarrays were performed.
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| Contributor(s) |
Hua F, Yu J |
| Citation(s) |
32694521 |
| Submission date |
Sep 15, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jiaojiao Yu |
| E-mail(s) |
yujiaojiao1989@126.com
|
| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
1 Xian Nong Tan Street
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA407416 |
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