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| Status |
Public on Dec 24, 2017 |
| Title |
expression data from human lung adenocarcinoma cells A549 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
GMDS is important for human lung adenocarcinoma progression, detailed molecular mechanisms remain elusive.
We used microarray to detail the global gene expression changes in A549 cells with GMDS knockdown as compared to control cells
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| Overall design |
A549 cells infected with lentivirus expressing either Scr-shRNA (n=3) or GMDS-shRNA (n=3) were cultured and then total RNA was extracted using Trizol reagents. RNA quantity and quality were determined using NanoDrop 2000 and Agilent Bioanalyzer 2100. For gene expression analysis, Affymetrix human GeneChip primeview was used according to the manufacturer’s instructions. In brief, GeneChip 3’ IVT Expression Kit was used for first-strand complementary DNA synthesis, double-stranded DNA template conversion, in vitro transcription for aRNA synthesis and labelling. Microarray hybridization, washing and staining were done using GeneChip Hybridization Wash and Stain Kit.
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| Contributor(s) |
Wang H |
| Citation(s) |
29843634 |
| Submission date |
Sep 22, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
HONG WANG |
| E-mail(s) |
18511244323@163.com
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| Organization name |
The Affiliated Hospital of Military Medical Sciences, The 307th Hospital of Chinese People’s Liberation Army
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| Department |
Department of Lung Cancer
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| Street address |
No.8 East Avenue, Fengtai District,
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| City |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (6)
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| GSM2790309 |
A549 cells with GMDS knockdown, biological rep1 |
| GSM2790310 |
A549 cells with GMDS knockdown, biological rep2 |
| GSM2790311 |
A549 cells with GMDS knockdown, biological rep3 |
| GSM2790312 |
A549 cells with scrambled knockdown, biological rep1 |
| GSM2790313 |
A549 cells with scrambled knockdown, biological rep2 |
| GSM2790314 |
A549 cells with scrambled knockdown, biological rep3 |
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| Relations |
| BioProject |
PRJNA411770 |
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