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| Status |
Public on Apr 13, 2018 |
| Title |
Analysis of microarray data reliability and pathway networks using experimental formalin-fixed paraffin-embedded tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We assessed the usability of microarrays, which base on formalin-fixed paraffin-embedded (FFPE) tissue.
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| Overall design |
We used CX3CR1-CreERT2+/–/iDTR+/– mice, which were injected with tamoxifen and diphtheria toxin to induce the depletion of microglia cells. Tissue was harvested 10 days post injection. We included 4 depleted mice as well as 4 control mice that received tamoxifen injections only. From each mouse brain, the motor cortex and the somatosensory cortex were microdissected.
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| Contributor(s) |
Brunner F, Wimmer I |
| Citation(s) |
29679021, 30385785 |
| Submission date |
Oct 05, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Isabella Wimmer |
| E-mail(s) |
isabella.wimmer@meduniwien.ac.at
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| Phone |
+4314016034222
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| Organization name |
Medical University of Vienna
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| Department |
Center for Brain Research
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| Lab |
Department of Neuroimmunology
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| Street address |
Spitalgasse 4
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (16)
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| GSM2804415 |
Control mouse, motor cortex 4 |
| GSM2804416 |
Control mouse, somatosensory cortex 1 |
| GSM2804417 |
Control mouse, somatosensory cortex 2 |
| GSM2804418 |
Control mouse, somatosensory cortex 3 |
| GSM2804419 |
Control mouse, somatosensory cortex 4 |
| GSM2804420 |
Microglia-depleted (day 10) mouse, motor cortex 1 |
| GSM2804421 |
Microglia-depleted (day 10) mouse, motor cortex 2 |
| GSM2804422 |
Microglia-depleted (day 10) mouse, motor cortex 3 |
| GSM2804423 |
Microglia-depleted (day 10) mouse, motor cortex 4 |
| GSM2804424 |
Microglia-depleted (day 10) mouse, somatosensory cortex 1 |
| GSM2804425 |
Microglia-depleted (day 10) mouse, somatosensory cortex 2 |
| GSM2804426 |
Microglia-depleted (day 10) mouse, somatosensory cortex 3 |
| GSM2804427 |
Microglia-depleted (day 10) mouse, somatosensory cortex 4 |
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| Relations |
| BioProject |
PRJNA413382 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE104634_RAW.tar |
77.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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