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| Status |
Public on Oct 12, 2017 |
| Title |
Expression data from APA+ and APA- progenitors and SSEA5+ human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human embryonic stem cells (hESC) can be differentiated into progenitors resembling trophoblast upon exposure to BMP4. Putative trophpblast progenitors express APA cell surface marker
Using FACS sorting and microarray we analysed the gene expression of APA-positive trophoblast progenitors, APA-negative progenitors and SSEA5-positive undifferentiated hESC and identified genes that contribute to the trophoblast fate
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| Overall design |
Undifferentiated hESC and hESC treated with BMP4 for 2.5 days (cell line H9) were FACS-sorted to isolate APA+, APA- and SSEA5+ cell populations. Sorted cells were subjected to RNA isolation and microarray protocol. The experiment was conducted using 3 independent differentiation experiments
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| Contributor(s) |
Shaposhnikov D, Krendl C |
| Citation(s) |
29078328 |
| Submission date |
Oct 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Dmitry Shaposhnikov |
| E-mail(s) |
dmitry.shaposhnikov@helmholtz-muenchen.de
|
| Organization name |
Helmholtz Center Munich
|
| Street address |
Ingolstädter Landstraße 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM2808386 |
Sorted APA-negative cell population, biological replicate 1 |
| GSM2808387 |
Sorted APA-negative cell population, biological replicate 2 |
| GSM2808388 |
Sorted APA-negative cell population, biological replicate 3 |
| GSM2808389 |
Sorted APA-positive cell population, biological replicate 1 |
| GSM2808390 |
Sorted APA-positive cell population, biological replicate 2 |
| GSM2808391 |
Sorted APA-positive cell population, biological replicate 3 |
| GSM2808392 |
Sorted SSEA5-positive undifferentiated hESC, biological replicate 1 |
| GSM2808393 |
Sorted SSEA5-positive undifferentiated hESC, biological replicate 2 |
| GSM2808394 |
Sorted SSEA5-positive undifferentiated hESC, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA413926 |
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