|
| Status |
Public on Oct 20, 2017 |
| Title |
Substoichiometric ribose methylations in spliceosomal snRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Sequencing-based profiling of ribose methylations is a new approach that allows for experiments adrressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.
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| |
|
| Overall design |
12 biological samples analysed in technical triplicates, in total 36 samples
|
| Web link |
http://pubs.rsc.org/en/content/articlelanding/2017/ob/c7ob02317k#!divAbstract
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| |
|
| Contributor(s) |
Krogh N, Kongsbak-Wismann M, Geisler C, Nielsen H |
| Citation(s) |
29048444 |
| Submission date |
Oct 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolai Krogh |
| E-mail(s) |
nicolaikj@sund.ku.dk
|
| Organization name |
University of Copenhagen
|
| Department |
Department of Cellular and Molecular Medicine
|
| Lab |
RNA Group - Prof. Henrik Nielsen
|
| Street address |
Blegdamsvej 3B
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA414213 |
| SRA |
SRP119915 |
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