 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 01, 2017 |
| Title |
MicroRNA Contents in Matrix Vesicles Produced by Growth Plate Chondrocytes Are Cell Maturation Dependent |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Chondrocytes at different maturation states in the growth plate produce matrix vesicles (MVs), membrane organelles found in the extracellular matrix, with a wide range of contents, such as matrix processing enzymes and receptors for hormones. We have shown that MVs harvested from growth zone (GC) chondrocyte cultures contain abundant small RNAs, including miRNAs. Here, we determined whether RNA also exists in MVs produced by less mature resting zone (RC) chondrocytes and, if so, whether it differs from the RNA in MVs produced by GC cells. Our results showed that RNA, small RNA specifically, was present in RC-MVs, and it was well-protected from RNase by the phospholipid membrane. A group of miRNAs was enriched in RC-MVs compared RC-cells, suggesting that miRNAs are selectively packaged into MVs. High throughput array and RNA sequencing showed that ~39% miRNAs were differentially expressed between RC-MVs and GC-MVs. Individual RT-qPCR also confirmed that miR-122-5p and miR-150-5p were expressed at significantly higher levels in RC-MVs compared to GC-MVs. This study showed that growth plate chondrocytes at different differentiation stages produce different MVs with different miRNA contents, further supporting extracellular vesicle miRNAs play a role as “matrisomes” that mediate the cell–cell communication in cartilage and bone development.
|
| |
|
| Overall design |
miRNA expression profiles of resting zone chondrocyte cells and matrix vesicles
|
| |
|
| Contributor(s) |
Lin Z, McClure MJ, Zhao J, Ramey AN, Asmussen N, Hyzy SL, Schwartz Z, Boyan BD |
| Citation(s) |
29483516 |
| Submission date |
Oct 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Barbara D. Boyan |
| E-mail(s) |
bboyan@vcu.edu
|
| Phone |
804 828 0190
|
| Organization name |
Virginia Commonwealth University
|
| Department |
School of Engineering
|
| Street address |
601 W. Main St.
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23284 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA416345 |
| SRA |
SRP122955 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106348_nat_bw2-ReadCount.tab.gz |
12.0 Kb |
(ftp)(http) |
TAB |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...