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| Status |
Public on Oct 30, 2018 |
| Title |
Transcriptional regulation of lipophorin receptors supports neuronal adaptation to chronic elevations of activity |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Activity-dependent modifications strongly influence neural development. However, molecular programs underlying their context and circuit-specific effects are not well understood. To study global transcriptional changes associated with chronic elevation of synaptic activity, we performed cell-type-specific transcriptome profiling of Drosophila ventral lateral neurons (LNvs) in the developing visual circuit and identified activity-modified transcripts that are enriched in neuron morphogenesis, circadian regulation, and lipid metabolism and trafficking. Using bioinformatics and genetic analyses, we validated activity-induced isoform-specific upregulation of Drosophila lipophorin receptors LpR1 and LpR2, the homologues of mammalian low-density-lipoprotein receptor (LDLR) family proteins. Furthermore, our morphological and physiological studies uncovered critical functions of neuronal LpRs in maintaining the structural and functional integrities in neurons challenged by chronic elevations of activity. Together, our findings identify LpRs as molecular targets for activity-dependent transcriptional regulation and reveal the functional significance of cell-type-specific regulation of neuronal lipid uptake in experience-dependent plasticity and adaptive responses.
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| Overall design |
LNv neuron-specific mRNA profiling in Drosophila larvae subjected to different visual experiences during development
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| |
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| Contributor(s) |
Yin J, Yuan Q |
| Citation(s) |
30380410 |
| Submission date |
Nov 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jun Yin |
| E-mail(s) |
jun.yin@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
NINDS
|
| Lab |
Dendrite Morphogenesis and Plasticity Unit
|
| Street address |
35 Convent Drive, Room 1B-1006
|
| City |
Bethesda |
| State/province |
Maryland (MD) |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA418460 |
| SRA |
SRP125033 |
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