|
| Status |
Public on Nov 30, 2017 |
| Title |
miR-975 overexpression effect on Drosophila cell lines |
| Organisms |
Drosophila melanogaster; Drosophila simulans; Drosophila virilis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We overexpressed the conspecific miR-975 in the cell lines derived from multiple Drosophila species (i.e. S2 cells from Drosophila melanogaster; ML82-19a cells from Drosophila simulans and WR-Dv-1 cells from Drosophila virilis). Comparison of the miR-975 effect between different cell lines provide insight into the rewiring of regulatory network through the evolution of new microRNA.
|
| |
|
| Overall design |
We examined conspecific miR-975 effect in three cell types. Empty vector (pUAST-only) was transfected as control. Two biological replicates were done for each treatment.
|
| |
|
| Contributor(s) |
Lyu Y, Liufu Z, Xiao J, Wu C, Tang T |
| Citation(s) |
34777478 |
| Submission date |
Nov 27, 2017 |
| Last update date |
Nov 18, 2021 |
| Contact name |
Yang Lyu |
| E-mail(s) |
yanglyu@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
109 Zina Pitcher Place
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48104 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL13306 |
Illumina HiSeq 2000 (Drosophila simulans) |
| GPL13313 |
Illumina HiSeq 2000 (Drosophila virilis) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA419952 |
| SRA |
SRP125694 |
Less...
More...