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| Status |
Public on Feb 05, 2019 |
| Title |
Transcriptomic analysis of KDM4B-mediated genes in AGS cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
KDM4B, an important epigenetic regulator of cell proliferation, metastasis and genome stability, is often overexpressed in gastric cancer. Notably, elevated expression of KDM4B is associated with a poor clinical outcome. A global transcriptomic analysis between KDM4B control and KDM4B-knockdown AGS cells without or with Helicobacter pylori challenge reveals differentially expressed genes involved in response to virus, multi-organism process, and response to stimulus, suggesting KDM4B as an inducible epigenetic factor under H. pylori challenge.
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| Overall design |
KDM4B control and knockdown cell lines were established in AGS cells based on the lentivirus system: pLKO (control) and shKDM4B (shRNA-specific RNA) lines. Global expression analysis was performed in AGS cells (pLKO vs. KDM4B-knockdown) uninfected or infected by H. pylori at an moi of 50 for 6 h. Microarray analysis was conducted by the National Health Research Institutes, using Affymetrix GeneChip Human Gene 2.0 ST Array (Affymetrix, Santa Clara, California, USA).
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| Contributor(s) |
Wu MC, Li YC, Wang WC |
| Citation(s) |
30683841 |
| Submission date |
Dec 05, 2017 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Meng-Chen Wu |
| E-mail(s) |
mengjhen@gapp.nthu.edu.tw
|
| Organization name |
National Tsing Hua University
|
| Department |
Institute of Molecular and Cellular Biology
|
| Lab |
Dr. Wen-Ching Wang
|
| Street address |
101, Section 2 Kuang Fu Road
|
| City |
Hsinchu city |
| ZIP/Postal code |
300 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA421157 |
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