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| Status |
Public on Dec 12, 2017 |
| Title |
Uterine Influences on Conceptus Development in Fertility-Classified Animals |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study relates to pregnancy outcome after assisted reproduction of fertility-classified cattle. The aim is to investigate how the uterine environment impacts and programs conceptus survival and development. The study found that ripple effects of dysregulated conceptus-endometrial interactions elicit post-elongation pregnancy loss in subfertile animals during the implantation period.
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| Overall design |
Heifer cows classified as high fertile (HF), subfertile (SF), or infertile (IF) were investigated. The RNA-seq analysis was performed for endometrium samples at day 17 of pregnancy. For comparison, non-pregnant cows were included in the analysis. RNA from conceptus of HF and SF pregnant animals (day 17) were also included in the RNA-seq analysis. A total of 25 endometrium samples (5 non-pregnant of each fertilty group, 5 pregnant HF, and 5 pregnant SF) and 27 conceptus samples (10 SF and 17 HF) were used in the RNA-seq analysis.
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| Contributor(s) |
Moraes JG, Behura SK, Geary TW, Hansen PJ, Neibergs HL, Spencer TE |
| Citation(s) |
29432175 |
| Submission date |
Dec 11, 2017 |
| Last update date |
Mar 13, 2018 |
| Contact name |
Thomas Spencer |
| E-mail(s) |
spencerte@missouri.edu
|
| Organization name |
University of Missouri
|
| Department |
Animal Sciences
|
| Lab |
ASRC 158
|
| Street address |
920 East Campus Drive
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
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| Samples (52)
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| Relations |
| BioProject |
PRJNA421885 |
| SRA |
SRP126481 |
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