|
| Status |
Public on Jun 20, 2018 |
| Title |
Chromatin remodeler CHD8 in CNS myelination and remyelination |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Other
|
| Summary |
Mutations in CHD8, an ATP-dependent chromodomain-helicase-DNA-binding protein 8, are known to cause a set of Autism Spectrum Disorders (ASD). We found that CHD8 is highly expressed in myelinating oligodendrocytes (OL) and their progenitors in the developing central nervous system (CNS). We performed CHD8 chIP-seq analysis in cultured oligodendrocytes.
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| |
|
| Overall design |
11 samples:
RNA-seq:2 Control Samples vs 2 cKO samples;
ChIP-seq: 2 Chd8samples, 1 chd7 sample;
H3K4me3 ChIP-seq: 1 control vs 1 Ch8cko;
ATAC-seq 1 control vs 1 Chd8cKO
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| |
|
| Contributor(s) |
Zhao C, Lu R |
| Citation(s) |
29920279 |
| Submission date |
Dec 11, 2017 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Richard Lu |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
CBDI
|
| Lab |
Lu Lab,T6.525
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (11)
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|
| Relations |
| BioProject |
PRJNA421937 |
| SRA |
SRP126504 |
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