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| Status |
Public on Apr 04, 2018 |
| Title |
Investigation of the gamma-tubulin binding site in the nuclear and mitochondrial DNA of cells in S-phase |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In a cell, g-tubulin establishes a cellular network of threads named g-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that g-strings have the ability to connect the cytoplasm and the mitochondrial DNA together. We also show that g-tubulin has a role in the maintenance of the mitochondrial meshwork and homeostasis as reduced levels of g-tubulin or impairment of its GTPase domain disrupts the mitochondria network and alters both their respiratory capacity and the expression of mitochondria-related genes. By contrast, reduced mitochondria number or increased protein levels of g-tubulin DNA-binding domain favour the association of g-tubulin with mitochondria. Our results demonstrate that g-tubulin is an important mitochondrial structural component that maintains the mitochondria meshwork, providing mitochondria with a cellular infrastructure. We propose that g-tubulin provides a cytoskeletal element that maintains mitochondria homeostasis and gives form to the mitochondria meshwork.
Keywords: Genome binding/occupancy profiling by high throughput sequencing
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| Overall design |
The experiment was performed in an MCF10A cell line and a total number of 16 samples were included. There are two samples per timepoint (one and two hours) and a whole cell lysate (WCE) is included as a control for each sample.
Please note that processed data was generated from two samples (replicates *.1 and *.2) and is linked to the first replicate (i.e. *1.1, *2.1) of each pair.
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| Contributor(s) |
Alvarado-Kristensson M, Lindström L |
| Citation(s) |
30271923 |
| Submission date |
Jul 13, 2018 |
| Last update date |
Jan 08, 2020 |
| Contact name |
Maria Alvarado Kristensson |
| E-mail(s) |
maria.alvarado-kristensson@med.lu.se
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| Organization name |
Lund University
|
| Street address |
59 Jan Waldenströms street
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| City |
Malmö |
| ZIP/Postal code |
20502 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
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| Samples (16)
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| Relations |
| SRA |
SRP126556 |
| BioProject |
PRJNA422088 |
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