|
| Status |
Public on Mar 18, 2019 |
| Title |
miR-7977 transfer alter the transcriptome in bone marrow mesenchymal stem cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Acute myeloid leukemia (AML) cells release abundant exosomal miR-7977 that transfer into bone marrow (BM) mesenchymal stromal cells (MSCs).
We have shown that exosomal miR-7977 was highly released from AML cells and was transferred into BM MSCs. It has been well known that a microRNA has multiple targets. In fact, miRDB predicted 633 targets. Based on these findings, control and miR-7977mimic were transferred into BM MSCs. Subsequently, alteration of transcriptome was analyzed to gain insight into the role of miR-7977 in bone marrow micro environment.
|
| |
|
| Overall design |
We performed transduction of control or miR-7977 mimic into MSCs, compared transcriptome in between control transduced (n=3) and miR-7977 transduced MSCs (n=3) using the Affymetrix Human Gene 2.0 ST platform.
|
| |
|
| Contributor(s) |
Kobune M, Yoshida M |
| Citation(s) |
30835743 |
| Submission date |
Dec 18, 2017 |
| Last update date |
Mar 18, 2019 |
| Contact name |
Masayoshi Kobune |
| E-mail(s) |
mkobune@sapmed.ac.jp
|
| Phone |
81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Hematology
|
| Street address |
Chuo-Ku, South-1, West-16
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8543 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA422770 |
Less...
More...