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| Status |
Public on Dec 13, 2020 |
| Title |
Piwi suppresses transcription of Brahma-dependent transposons via Maelstrom in ovarian somatic cells |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Drosophila Piwi associates with PIWI-interacting RNAs (piRNAs) and represses transposons transcriptionally through heterochromatinization; however, this process is poorly understood. Here, we identify Brahma (Brm), the core adenosine triphosphatase of the SWI/SNF chromatin remodeling complex, as a new Piwi interactor, and show Brm involvement in activating transcription of Piwi-targeted transposons before silencing. Bioinformatic analyses indicated that Piwi, once bound to target RNAs, reduced the occupancies of SWI/SNF and RNA polymerase II (Pol II) on target loci, abrogating transcription. Artificial piRNA-driven targeting of Piwi to RNA transcripts enhanced repression of Brm-dependent reporters compared with Brm-independent reporters. This was dependent on Piwi cofactors, Gtsf1/Asterix (Gtsf1), Panoramix/Silencio (Panx), and Maelstrom (Mael), but not Eggless/dSetdb (Egg)–mediated H3K9me3 deposition. The lambdaN-box B–mediated tethering of Mael to reporters repressed Brm-dependent genes in the absence of Piwi, Panx, and Gtsf1. We propose that Piwi, via Mael, can rapidly suppress transcription of Brm-dependent genes to facilitate heterochromatin formation.
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| Overall design |
Transcriptome-wide examination of gene expression changes in Piwi-, Mael-, Brm-depleted OSCs. Genome-wide examination of changes in chromatin binding levels of Drosophila SWI/SNF complexes including Brm in Drosophila ovaries and OSCs.
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| Contributor(s) |
Onishi R, Sato K, Murano K, Negishi L, Siomi H, Siomi MC |
| Citation(s) |
33310860 |
| Submission date |
Dec 20, 2017 |
| Last update date |
Dec 22, 2020 |
| Contact name |
Kaoru Sato |
| E-mail(s) |
k_sato@tmig.or.jp
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| Organization name |
Tokyo Metropolitan Institute of Gerontology
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| Street address |
35-2 Sakae-cho, Itabashi-ku
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| City |
Tokyo |
| ZIP/Postal code |
1730015 |
| Country |
Japan |
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| Platforms (2) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA423181 |
| SRA |
SRP127227 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE108329_171220-Piwi-Brm_transcripts.txt.gz |
1.1 Mb |
(ftp)(http) |
TXT |
| GSE108329_Brm-ChIPseq-processed_data_file.csv.gz |
1.7 Mb |
(ftp)(http) |
CSV |
| GSE108329_RAW.tar |
158.1 Mb |
(http)(custom) |
TAR (of BEDGRAPH) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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