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| Status |
Public on Apr 01, 2018 |
| Title |
Differential 3’ Processing of Specific Transcripts Expands Regulatory and Protein Diversity Across Neuronal Cell Types |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Alternative polyadenylation (APA) regulates mRNA translation, stability, and protein localization. However, it is unclear to what extent APA regulates these processes uniquely in specific cell types. Using a new technique, cTag-PAPERCLIP, we discovered significant differences in APA between the principal types of mouse cerebellar neurons, the Purkinje and granule cells, as well as between proliferating and differentiated granule cells. Transcripts that differed in APA in these comparisons were enriched in key neuronal functions and many differed in coding sequence in addition to 3’UTR length.
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| Overall design |
Examination of Pabpc1-GFP bound RNA in cerebellar granule in Purkinje cells and cerebellar granule cells during development using a cTag-PAPERCLIP. RNA-seq of RNA from cerebellar granule cells at two developmental timepoints to compare with cTag-PAPERCLIP data.
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| Contributor(s) |
Jereb S, Hwang H, Fak JJ, Hatten ME, Darnell RB |
| Citation(s) |
29578408 |
| Submission date |
Dec 22, 2017 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Saša Jereb |
| Organization name |
The Rockefeller University
|
| Department |
Laboratory of Molecular Neuro-Oncology
|
| Lab |
Robert B. Darnell Lab
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA427352 |
| SRA |
SRP127471 |
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