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Series GSE108685 Query DataSets for GSE108685
Status Public on Jan 03, 2020
Title Genome-wide blood transcriptional profiling in human endotoxemia given human adipose-derived mesenchymal stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Mesenchymal stem cells (MSCs) can modulate various immune responses implicated in the pathogenesis of sepsis. Intravenous injection of lipopolysaccharide (LPS) into healthy subjects represents a model with relevance for the host response to sepsis.
 
Overall design To determine the effect of MSCs on the early inflammatory response to intravenous LPS we conducted a randomized study in 32 healthy subjects with 4 treatment arms: placebo or adipocyte-derived MSCs intravenously at either 250.000, 1 million or 4 million cells/kg; all subjects received LPS intravenously (2 ng/kg) one hour after the end of MSC infusion. PAXgene blood RNA was isolated before and at 2, 4, 6 and 24 hours after LPS injection. Through the use of genome-wide transcriptional profiling we aimed to identify features of the systemic host transcriptional response to E.coli LPS endotoxemia that were altered due to mesenchymal stem cell administration. Moreover, biological pathway analysis was used to tease out the most relevant biological units.
 
Contributor(s) Scicluna BP, van der Poll T
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Submission date Jan 03, 2018
Last update date Jan 05, 2020
Contact name Brendon Scicluna
E-mail(s) brendon.scicluna@um.edu.mt
Organization name University of Malta
Street address Msida campus
City Msida
ZIP/Postal code MSD2020
Country Malta
 
Platforms (1)
GPL13667 [HG-U219] Affymetrix Human Genome U219 Array
Samples (190)
GSM2910974 human_endotoxemia_2ng/kg_Ecoli_LPS [19_05_2015_A01_100114]
GSM2910975 human_endotoxemia_2ng/kg_Ecoli_LPS [19_05_2015_A02_100086]
GSM2910976 human_endotoxemia_2ng/kg_Ecoli_LPS [19_05_2015_A03_100056]
Relations
BioProject PRJNA428290

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108685_RAW.tar 368.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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