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| Status |
Public on Nov 21, 2023 |
| Title |
Fam46a is a regulator for gene expression in secretory tissues and plays an important role in development and homeostasis [FAM46A-shRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In early embryonic development, the organiser functions in multiple developmental processes, including neural induction, dorsoventral patterning of the mesoderm, and formation of the axial mesoderm. Chordin is a central molecule for the activity of the Spemann’s organiser and abundantly secreted from the Spemann’s organiser in Xenopus laevis. However, so far, a whole picture of the gene expression profile regulated by Chordin has not been examined. Here, we perform microarray analysis of chordin overexpression and identify fam46a as an upregulated gene. We demonstrate that fam46a is essential for Xenopus laevis early embryonic development as well as gene induction during cell differentiation in mammalian cell culture models. fam46a is preferentially expressed in tissues with high secretory ability. In Xenopus laevis, fam46a is required for normal neural tube closure and expression of epidermal markers, neural markers and mesodermal markers. In the human Caco-2 cell model, FAM46A is localised in the nucleus and cytoplasm, and regulates gene induction during cell differentiation. In the mouse intestinal organoid model, Fam46a is required for survival of organoids as well as enterocyte differentiation. These results indicate that fam46a is a regulator for gene expression in secretory cells during cell differentiation in early embryonic development and adult homeostasis.
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| Overall design |
To analyse which genes were affected by FAM46A depletion in Caco-2 cells, we performed microarray analysis on Caco-2 cells infected with shRNA-expressing lentivirus.
Caco-2 cells were infected with lentivirus expressing scramble shRNA, FAM46A-shRNA#2 or FAM46A-shRNA#4.
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| Contributor(s) |
Suzuki T, Shintani C, Araki T, Miyatake K, Kusakabe M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 03, 2018 |
| Last update date |
Nov 21, 2023 |
| Contact name |
Toshiyasu Suzuki |
| E-mail(s) |
T.Suzuki@beatson.gla.ac.uk
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| Organization name |
Kyoto University
|
| Department |
Department of Cell and Developmental Biology, Graduate school of Biostudies
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| Street address |
Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2911288 |
Caco-2 cells expressing scramble shRNA, biological rep1 |
| GSM2911289 |
Caco-2 cells expressing scramble shRNA, biological rep2 |
| GSM2911290 |
Caco-2 cells expressing FAM46A-shRNA#2, biological rep1 |
| GSM2911291 |
Caco-2 cells expressing FAM46A-shRNA#2, biological rep2 |
| GSM2911292 |
Caco-2 cells expressing FAM46A-shRNA#4, biological rep1 |
| GSM2911293 |
Caco-2 cells expressing FAM46A-shRNA#4, biological rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE108692 |
Fam46a is a regulator for gene expression in secretory tissues and plays an important role in development and homeostasis |
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| Relations |
| BioProject |
PRJNA428304 |
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