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| Status |
Public on Aug 13, 2018 |
| Title |
Expression data from erythroblast (CD71 and GPA positive cells) derived from fetal liver and adult blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The fetal hemoglobin (HbF) levels were 95.4 ± 1.5% and 4.4 ± 0.2% in fetal liver-derived and adult blood derived-cultured erythrocytes (n=5), respectively. Following RNA isolation from the CD71 high/GPA positive erythroblasts, gene expression analyses were performed using Affymetrix Human Gene 2.0 ST Array.
The Affymetrix raw data files of microarray were preprocessed using robust multi-array average method for background correction, log-transformation, and quantile normalization. Differential gene expression between fetal liver derived- and adult peripheral blood derived-erythroblasts was determined using moderated t-statistics implemented in the Limma package. We found the 1366 genes were upregulated by more than 1.5-fold change with adjusted p-value less than 0.05 in fetal liver derived-erythroblasts.
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| Overall design |
The fetal liver-derived and adult blood derived-cultured erythrocytes (n=5), respectively. Gene expression analyses were performed using Affymetrix Human Gene 2.0 ST Array. The Affymetrix raw data files of microarray were preprocessed using robust multi-array average method for background correction, log-transformation, and quantile normalization. Differential gene expression between fetal liver derived- and adult peripheral blood derived-erythroblasts was determined using moderated t-statistics implemented in the Limma package.
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| Contributor(s) |
Tangprasittipap A, Kaewprommal P, Sripichai O, Sathirapongsasuti N, Satirapod C, Shaw PJ, Piriyapongsa J, Hongeng S |
| Citation(s) |
30186694 |
| Submission date |
Jan 15, 2018 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Pavita Kaewprommal |
| E-mail(s) |
pavita.kae@nstda.or.th
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| Organization name |
NSTDA
|
| Department |
NBT
|
| Lab |
DATA
|
| Street address |
113
|
| City |
Khlong Nung |
| State/province |
Pathum Thani |
| ZIP/Postal code |
12120 |
| Country |
Thailand |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA430073 |
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