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| Status |
Public on Mar 29, 2018 |
| Title |
Development of plasma cell-prone germinal center B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Higher- or lower-affinity GC B cells are directed to plasma cell or recycling GC cell fates; however, how commitment to the plasma cell fate takes place is unclear. By using the level of Bcl6 as a marker, we found that a population of light zone (LZ) GC cells, Bcl6loCD69hi with IRF4 and higher-affinity BCRs or Bcl6hiCD69hi with lower- affinity BCRs, favored the plasma cell or recycling GC cell fate, respectively. Mechanistically, CD40 acted as a dose-dependent regulator for Bcl6loCD69hi cell formation. Furthermore, we found that ICAM1 and SLAM levels on Bcl6loCD69hi cells were higher than on Bcl6hiCD69hi cells, thereby affording more stable TFH-GC B cell contacts, while attenuating this contact down-regulated IRF4. These data support the model that commitment to the plasma cell begins in the GC, and suggest that stability and possibly duration of TFH-GC B cell contacts are key for formation of plasma cell-prone precursor GC cells.
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| |
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| Overall design |
mRNA profiles of GC B cell subsets and GC-derived plasmablast were generated by barcode-based digital RNA sequencing.
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| |
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| Contributor(s) |
Ise W, Shiroguchi K, Kurosaki T |
| Citation(s) |
29669250 |
| Submission date |
Jan 26, 2018 |
| Last update date |
Jan 27, 2019 |
| Contact name |
Eri Nishiyama |
| E-mail(s) |
eri.nishiyama@riken.jp
|
| Organization name |
Riken
|
| Department |
Center for Biosystems Dynamics Research
|
| Lab |
Laboratory for Prediction of Cell Systems Dynamics
|
| Street address |
6-2-3 Furuedai
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0874 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA431808 |
| SRA |
SRP131526 |
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