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| Status |
Public on Feb 21, 2020 |
| Title |
Intergrated epigenetics, transcriptomics and metabolomics to analyze the mechanism of Benzo[a]pyrene neurotoxicity in hippocampus |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: The aim of the study was to investigate the global effect of B[a]P on DNA methylation, non-coding RNAs (ncRNAs) and coding RNAs expression in the rat hippocampus.
Methods: High-throughput sequencing was applied to assess hippocampus DNA methylation, ncRNAs and coding RNAs expression.
Results: Exposure to B[a]P could influence the levels of DNA methylation, and the expression profiles of ncRNAs and mRNAs in rats.
Conclusions: B[a]P could bring on the epigenetics changes as well as the transcriptomics changes and further affected gene expression.
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| Overall design |
B[a]P was dissolved in corn oil. The B[a]P-treated rat was administered by oral gavage each day in a dose of 2.0mg/kg B[a]P and the control rat was also administered by oral gavage but with equivalent volume of corn oil. Then high-throughput sequencing was performed for B[a]P-treated and control rat hippocampal.
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| Contributor(s) |
Wang J, Li C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 01, 2018 |
| Last update date |
Feb 22, 2020 |
| Contact name |
Jing Wang |
| E-mail(s) |
592214395@qq.com
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| Organization name |
Chongqing Medical University
|
| Street address |
Yuzhong District Petroleum Road on the 1st
|
| City |
Chongqing |
| ZIP/Postal code |
400016 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA432500 |
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