|
| Status |
Public on Dec 31, 2020 |
| Title |
Analysis of gene expression in co-culture of PBMCs with ovarian cancer cell lines by High-Throughput Sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
(Objectives) The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on ovarian cancer cell lines.
(Methods) Transcription profiles of PBMC (control) and PBMC co-cultured with ovarian cancer cell lines (treatment) were generated by deep sequencing, in triplicate, using Illumina HiSeq X Ten.
(Results and Conclusions) Transcriptome analysis of PBMCs has revealed the expression change when co-cultured with EOC cell lines with significantly in up-regulated genes; CDKN1B, GIMAP8 and SNN. The qRT-PCR validation have indicated that GIMAP8 was high expressed in EOC patients when compared with healthy female. We summarized that PBMCs were changed their expression as a result of paracrine signals from ovarian cancer cells.
|
| |
|
| Overall design |
Transcription profiles of PBMC (control) and PBMC co-cultured with cancer (treatment) were generated by deep sequencing, using Illumina HiSeq 4000.
|
| Web link |
https://doi.org/10.4172/1948-5956.1000588
|
| |
|
| Contributor(s) |
Sonthi N, Kitkumthorn N, Mutirangura A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 16, 2018 |
| Last update date |
Dec 31, 2020 |
| Contact name |
Nakarin Kitkumthorn |
| E-mail(s) |
nakarinkitkumthorn@gmail.com
|
| Organization name |
Mahidol University
|
| Department |
Oral Biology
|
| Street address |
Yothee road
|
| City |
Bangkok |
| ZIP/Postal code |
10400 |
| Country |
Thailand |
| |
|
| Platforms (1) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA434447 |
| SRA |
SRP133005 |
Less...
More...