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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 05, 2018 |
| Title |
Expression data of hPSCs differentiated into Paraxial mesoderm |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Stem cell-derived tissues have wide potential for modelling developmental and pathological processes as well as cell-based therapy. However, it has proven difficult to generate several key cell types in vitro, including skeletal muscle. In vertebrates, skeletal muscles derive during embryogenesis from the presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions for the differentiation of monolayer cultures of human pluripotent stem (hPSC) cells into PSM-like cells without the introduction of transgenes or cell sorting. We differentiated human PSCs in serum-free medium supplemented with Chir99021 only (C medium) or with also the Bmp inhibitor LDN193189 (CL medium). In vivo, the PSM cells are first expressing MSGN1 (posterior PSM marker) and then mature to express Pax3 (anterior PSM marker). After 4-5 days of differentiation of hPSCs, MSGN1-positive cells were FACS-sorted and their transcriptome analyzed.
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| Overall design |
Human PSCs differentiated for 0, 4-5 days in serum-free medium containing a Chir (C medium) or Chir and Ldn (CL medium) to study paraxial mesoderm in vitro
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| Contributor(s) |
Tanoury ZA, Tassy O, Chal J |
| Citation(s) |
29555813 |
| Submission date |
Mar 05, 2018 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Jerome Chal |
| Organization name |
Brigham and Women's Hospital
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| Street address |
77 ave Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM3029953 |
hPSCs hMSGN1-Venus -positive at day4-5, Chir (C medium) differentiation, biological rep1 |
| GSM3029954 |
hPSCs hMSGN1-Venus -positive at day4-5, Chir (C medium) differentiation, biological rep2 |
| GSM3029955 |
hPSCs hMSGN1-Venus -positive at day4-5, Chir (C medium) differentiation, biological rep3 |
| GSM3029956 |
hPSCs hMSGN1-Venus -positive at day4-5, Chir/Ldn (CL medium) differentiation, biological rep1 |
| GSM3029957 |
hPSCs hMSGN1-Venus -positive at day4-5, Chir/Ldn (CL medium) differentiation, biological rep2 |
| GSM3029958 |
hPSCs hMSGN1-Venus -positive at day4-5, Chir/Ldn (CL medium) differentiation, biological rep3 |
| GSM3029959 |
hPSCs undifferentiated at day0, biological rep1 |
| GSM3029960 |
hPSCs undifferentiated at day0, biological rep2 |
| GSM3029961 |
hPSCs undifferentiated at day0, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE39615 |
Transcriptomic landscape of developing Presomitic Mesoderm (PSM) from Tailbud to somite in E9.5 mouse embryo and in in vitro differentiated Paraxial mesoderm derived from mouse embryonic stem cells (mESCs). |
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| Relations |
| BioProject |
PRJNA436908 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE111391_RAW.tar |
76.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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