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| Status |
Public on Mar 13, 2018 |
| Title |
Effect of transgenic RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We compared four transcription factor knockdowns using transgenic RNAi expressed in the larval fat body. FOXO, Tfb1, p53, and Stat92E-dependent gene expression in the Drosophila fat body was quantified on control and high-sugar diets in order to generate expression profiles via RNA-seq. These expression data were used to build a gene regulatory network to predict novel roles for these and other genes during caloric overload.
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| Overall design |
Control and fat body-expressed transcription factor RNAi Drosophila were reared on control (0.15M sucrose) and high-sugar (0.7M or 1M sucrose) diets until the wandering stage. Fat bodies were isolated and RNA extracted to determine the effects of diet on gene expression using Illumina RNA-seq.
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| Contributor(s) |
Musselman L, Baranski T, Maier E, Brent M |
| Citation(s) |
29487137 |
| Submission date |
Mar 12, 2018 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Laura Palanker Musselman |
| E-mail(s) |
palankl@hotmail.com
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| Organization name |
Binghamton University
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| Department |
Biological Sciences
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| Lab |
S3 room 210
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| Street address |
4400 Vestal Parkway East
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| City |
Binghamton |
| State/province |
New York |
| ZIP/Postal code |
13902 |
| Country |
USA |
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| Platforms (1) |
| GPL11203 |
Illumina Genome Analyzer IIx (Drosophila melanogaster) |
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| Samples (62)
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| Relations |
| BioProject |
PRJNA437974 |
| SRA |
SRP134974 |
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