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| Status |
Public on Mar 14, 2019 |
| Title |
Next Generation RNA sequencing of 30 human liver tissues at different developmental stages (10 each of Fetal, Pediatric and Adult) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
miRNAs have emerged as key regulators of gene expression. Here we sequenced mRNA from 30 human liver samples to identify genetic polymorphisms in predicted miRNA binding regions that show allele-specific gene expression. These genetic variants will be functionally tested for miRNA-mediated regulation.
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| Overall design |
Standard methods were used for RNA-sequencing library construction, EXBead preparation, and Next-Generation sequencing, based on the protocol provided for the Life Technologies SOLiD4 system. Briefly, 2μg of total RNA per sample of 30 human liver samples (fetal, pediatric, and adult; n=10) were used for library preparation. The rRNA was depleted using RiboMinus Eukaryote Kit for RNA-Seq.
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| Contributor(s) |
Skaar TC, Liu Y |
| Citation missing |
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| Submission date |
Mar 14, 2018 |
| Last update date |
Mar 14, 2019 |
| Contact name |
Yunlong Liu |
| E-mail(s) |
yunliu@iu.edu
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| Organization name |
Indiana University
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| Street address |
410 west 10th street
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| City |
Indianapolis |
| ZIP/Postal code |
46202 |
| Country |
USA |
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| Platforms (1) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA438348 |
| SRA |
SRP135703 |
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