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| Status |
Public on Oct 07, 2018 |
| Title |
The effect of inhibition of arginine methylation in human FOXP3-transduced T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The forkhead box transcription factor 3 (FOXP3) plays a pivotal role in the suppressive function of regulatory T cells. Protein function is diversely controlled by posttranslational mechanisms as well as mRNA expression levels. In this study, we demonstrate that FOXP3 undergoes arginine methylation by interacting with the protein arginine methyltransferase 1 (PRMT1).
The inhibition of arginine methylation conferred gene expression profiles representing type I helper T cells to FOXP3+ T cells, which resulted in attenuated suppressive activity. A methylation-defective mutant of FOXP3 displayed less potent activity to suppress xenogeneic graft-versus-host disease in vivo.
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| Overall design |
Human CD4+ T cells derived from three different donors were transduced with control DNGFR or FOXP3-DNGFR and cultured in the presence of DMSO or MS023 (type I PRMT inhibitor). The DNGFR+ cells were isolated on day 7, and the gene expression profiles were analyzed using the Affymetrix PrimeView™ Human Gene Expression Array.
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| Contributor(s) |
Kagoya Y, Hirano N |
| Citation(s) |
30318155 |
| Submission date |
Mar 23, 2018 |
| Last update date |
Jan 06, 2019 |
| Contact name |
Yuki Kagoya |
| Organization name |
Keio University
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| Department |
School of Medicine
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| Lab |
Division of Tumor Immunolgy, Institute for Advanced Medical Research
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| Street address |
35 Shinanomachi, Shinjuku
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| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (9)
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| GSM3064806 |
Control DNGFR-transduced CD4+ T cells, rep 1 |
| GSM3064807 |
Control DNGFR-transduced CD4+ T cells, rep 2 |
| GSM3064808 |
Control DNGFR-transduced CD4+ T cells, rep 3 |
| GSM3064809 |
FOXP3-transduced CD4+ T cells treated with DMSO, rep 1 |
| GSM3064810 |
FOXP3-transduced CD4+ T cells treated with DMSO, rep 2 |
| GSM3064811 |
FOXP3-transduced CD4+ T cells treated with DMSO, rep 3 |
| GSM3064812 |
FOXP3-transduced CD4+ T cells treated with MS023, rep 1 |
| GSM3064813 |
FOXP3-transduced CD4+ T cells treated with MS023, rep 2 |
| GSM3064814 |
FOXP3-transduced CD4+ T cells treated with MS023, rep 3 |
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| Relations |
| BioProject |
PRJNA445451 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112269_RAW.tar |
22.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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