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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 02, 2018 |
| Title |
Expression data from healthy murine bronchial epithelial cells and syngeneic murine lung squamous carcinoma (LUSC) from DBA2 mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The process of lung squamous carcinoma tumorigenesis and metastasis is poorly characterized. Additionally, few models of this process exist in an immune-competent context. In order to address this problem, we utilized the KLN-205 lung squamous carcinoma cell lines that is derived from carcinogen exposure in DBA2 mice.
We used healthy bronchial epithelial cells from adult DBA2 mice as a healthy control. Following an in vivo selection strategy to enrich for metastatic subclones (LN2-2, LN4-2, LN4K1), we used Affymetrix murine microarrays to study differentially expressed genes as the cells go from heathy, to tumorigenic but poorly metastatic to tumorigenic and highly metastatic (LN4K1).
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| Overall design |
Murine bronchial epithelial cells, KLN-205 and its metastatic subclones were grown to 60-80% confluence for RNA extraction and hybridization on Affymetrix microarrays. Then, differentially expressed genes were assessed as cells progressed from healthy, to tumorigenic to both tumorigenic and strongly metastatic (LN4K1).
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| Contributor(s) |
Porrello A, Pecot CV |
| Citation(s) |
29777108 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA215075 |
Immune Regulation of Lung Squamous Metastasis |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Chad V Pecot |
| P30 DK065988 |
Cell Models Core |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Scott H Randell |
| R35 CA197684 |
IKK/NF-kappaB Signaling in Cancer: Therapy, Resistance, and Tumor Initiating Cells |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
ALBERT Sidney BALDWIN |
| R01 HL126974 |
Fibrinogen and Factor XIII in Venous Thrombosis |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Alisa S Wolberg |
| F31 HL139100 |
Contribution of Plasma and Platelet Factor XIII to Venous Thrombosis |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Sravya Kattula |
| T32 CA196589 |
CAROLINA CANCER NANOTECHNOLOGY TRAINING PROGRAM (C-CNTP) |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
ALEXANDER V KABANOV |
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| Submission date |
Apr 01, 2018 |
| Last update date |
May 28, 2018 |
| Contact name |
Alessandro Porrello |
| E-mail(s) |
alessandro.porrello@gmail.com
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| Organization name |
University of North Carolina at Chapel Hill
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| Department |
Lineberger Comprehensive Cancer Center
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| Street address |
450 West Drive
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| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (16)
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| GSM3073264 |
Murine bronchial epithelial cells, biological rep1 |
| GSM3073265 |
Murine bronchial epithelial cells, biological rep2 |
| GSM3073266 |
Murine bronchial epithelial cells, biological rep3 |
| GSM3073267 |
Murine bronchial epithelial cells, biological rep4 |
| GSM3073268 |
KLN-205, murine LUSC cell line, biological rep1 |
| GSM3073269 |
KLN-205, murine LUSC cell line, biological rep2 |
| GSM3073270 |
KLN-205, murine LUSC cell line, biological rep3 |
| GSM3073271 |
KLN-LN2-2, sub-clone of KLN-205, biological rep1 |
| GSM3073272 |
KLN-LN2-2, sub-clone of KLN-205, biological rep2 |
| GSM3073273 |
KLN-LN2-2, sub-clone of KLN-205, biological rep3 |
| GSM3073274 |
KLN-LN4-2, sub-clone of KLN-205, biological rep1 |
| GSM3073275 |
KLN-LN4-2, sub-clone of KLN-205, biological rep2 |
| GSM3073276 |
KLN-LN4-2, sub-clone of KLN-205, biological rep3 |
| GSM3073277 |
KLN-LN4K1, sub-clone of KLN-205, biological rep1 |
| GSM3073278 |
KLN-LN4K1, sub-clone of KLN-205, biological rep2 |
| GSM3073279 |
KLN-LN4K1, sub-clone of KLN-205, biological rep3 |
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| Relations |
| BioProject |
PRJNA448337 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112585_RAW.tar |
74.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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