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| Status |
Public on Aug 20, 2018 |
| Title |
RNA-seq analysis to assess transcriptional effects of Rp mutations in wing imaginal discs and their dependence on Xrp1 |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Since the putative transcription factor Xrp1 gene is required for many aspects of Rp+/- phenotypes, we sought to define what transcriptional changes occur in Rp+/- wing discs and their dependence on Xrp1. We identified 257 genes whose transcripts were significantly altered in the same direction in two Rp+/- genotypes, most of which depended on Xrp1. The results show that Xrp1 regulates a significant transcriptional response in Rp+/- genotypes.
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| Overall design |
12 samples with wildtype (named as frt82_w) in triplicate, one type of Rp+/- (named as M67_w) in triplicate, and the other type of Rp+/- (named as M95_w) in triplicate, as well as RpS3+/- Xrp1+/- (named as M2-73_M95) in triplicate
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| Contributor(s) |
Kiparaki M, Blanco J, Folgado V, Ji Z, Kumar A, Rimesso G, Baker N |
| Citation(s) |
30078730, 31909714, 35179490 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM120451 |
Ribosomes and Growth Regulation |
ALBERT EINSTEIN COLLEGE OF MEDICINE |
Nicholas E Baker |
|
| Submission date |
Apr 09, 2018 |
| Last update date |
Mar 30, 2022 |
| Contact name |
Nicholas Baker |
| E-mail(s) |
Nicholas.baker@einsteinmed.org
|
| Phone |
7184302854
|
| Organization name |
albert einstein college of medicine
|
| Department |
genetics
|
| Street address |
1300 Morris Park Avenue
|
| City |
bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA449346 |
| SRA |
SRP139048 |
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