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| Status |
Public on Apr 18, 2018 |
| Title |
Genome-wide maps of linker histone H1 lysine 85 acetylation in HCT116 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We identify acetylation of linker histone H1 lysine 85 as a novel histone acetylation mark, which regulates chromatin structure and genome stability upon DNA damage. By performing a chromatin immunoprecipitation and deep sequencing assay, we identified the genome-wide distribution patterns of H1 lysine 85 acetylation in HCT116 cells. We found that H1K85ac largely bound intergenic regions (53%) and gene bodies (39%), whereas only 7% H1K85ac bound regions 2 kb upstream of the transcription start sites (TSSs). As the proportion of H1K85ac bound to potential promoter regions was not significantly different to that of the random peaks (~6%), these data suggest that H1K85ac may not be mainly involved in general transcriptional regulation.
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| Overall design |
Examination of H1 lysine 85 acetylation in HCT116 cells
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| Contributor(s) |
Li Y, Li Z |
| Citation(s) |
29982688 |
| Submission date |
Apr 17, 2018 |
| Last update date |
Feb 21, 2019 |
| Contact name |
Zhiming Li |
| E-mail(s) |
lizhiming@bjmu.edu.cn
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| Phone |
18682369120
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| Organization name |
Peking University Health Science Center
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| Street address |
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA450529 |
| SRA |
SRP140615 |
