|
| Status |
Public on Apr 21, 2021 |
| Title |
Small RNA seq in ALKBH3 stable over expresion and control HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Total RNA (100 μg) were denatured at 70 °C for 5 min and separated by a 15% TBE-Urea gel with 20/100 or 10/60 oligo length standard ladder (Integrated DNA Technologies, Coralville, IA). After visualized by SYBR Gold (Ref. S11494 from Life Technologies), tRFs (15~50 nt) in gels were cut according to sizes and recovered by small RNA PAGE recovery kit (Zymo Research, Irvine, CA, USA). The recovered tRFs was used to establish the library by NEB small RNA library preparation kit (E7330S).
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| |
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| Overall design |
Examination of small RNA difference in ALKBH3 stable over expresion and control HeLa cells
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| |
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| Contributor(s) |
Wang H, Luo G |
| Citation missing |
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| Submission date |
Apr 21, 2018 |
| Last update date |
Apr 21, 2021 |
| Contact name |
zhike lu |
| E-mail(s) |
zhikelu@gmail.com
|
| Organization name |
University of Chicago
|
| Department |
department of chemistry
|
| Street address |
5801 South Ellis Avenue
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (4)
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|
| Relations |
| BioProject |
PRJNA451300 |
| SRA |
SRP141723 |
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