 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 27, 2021 |
| Title |
Myoepithelial cell perturbations in BRCA mutation carriers and in DCIS (ductal carcinoma in situ) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Third-party reanalysis
|
| Summary |
We describe the gene expression profiles and enhancer landscape of normal myoepithelial cells and perturbations of these in BRCA1 and BRCA2 mutation carriers and in DCIS. We identified a myoepithelial transcription regulatory network orchestrated by p63 and TCF7 and defined the genomic targets of these transcription factors by ChIP-seq. While the majority of myoepithelial cells co-express p63 and TCF7 in normal breast of healthy women, the frequency of these cells is significantly lower in BRCA1 mutation carriers and in DCIS. Downregulation of p63 in MCF10DCIS cells leads to loss of myoepithelial cells and invasive tumors, whereas overexpression of TCF7 enhances tumor growth. Our findings suggest that loss of normal myoepithelial cell function facilitates in situ to invasive carcinoma transition and it may also enhance tumor initiation in BRCA mutation carriers.
|
| |
|
| Overall design |
Normal myoepithelial cells and perturbations of these in BRCA1 and BRCA2 mutation carriers and in DCIS
|
| |
|
| Contributor(s) |
Ding L, Su Y, Qiu X, Ekram M, Huh SJ, Bloushtain-Qimron N, Harper NW, Jovanovic B, Hines WC, Zi X, Merino VF, Choudhury S, Ethington G, Panos L, Grant M, Rosson GD, Clark J, Herlihy W, Au A, Richardson AL, Argani P, Hwang ES, Fan R, Allred DC, Bobolis K, Kleer C, Blum JL, Long H, Sukumar S, Park SY, Garber JE, Yao J, Bissell M, Polyak K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 27, 2018 |
| Last update date |
Apr 28, 2021 |
| Contact name |
Chunru Lin |
| E-mail(s) |
CLin2@mdanderson.org
|
| Phone |
713-745-3226
|
| Organization name |
M. D. Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (15)
|
|
| Relations |
| Reanalysis of |
GSM793369 |
| Reanalysis of |
GSM793372 |
| Reanalysis of |
GSM793373 |
| Reanalysis of |
GSM793371 |
| Reanalysis of |
GSM793370 |
| Reanalysis of |
GSM793374 |
| BioProject |
PRJNA454004 |
| SRA |
SRP143501 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE113795_GSM793369_N35_CD10.mapped.by.SAGE.genie.Best.Gene.txt.gz |
106.4 Kb |
(ftp)(http) |
TXT |
| GSE113795_GSM793369_N40_CD10.txt.gz |
8.4 Mb |
(ftp)(http) |
TXT |
| GSE113795_GSM793370_N48_CD10.mapped.by.SAGE.genie.Best.Gene.txt.gz |
106.6 Kb |
(ftp)(http) |
TXT |
| GSE113795_GSM793370_N_CD10+_CD44+_N102_FC00297_s8_LIB002171.txt.gz |
12.0 Mb |
(ftp)(http) |
TXT |
| GSE113795_GSM793371_N43_CD10.mapped.by.SAGE.genie.Best.Gene.txt.gz |
107.2 Kb |
(ftp)(http) |
TXT |
| GSE113795_GSM793371_N_CD10+_CD44-_N102_FC00297_s7_LIB002170.txt.gz |
23.6 Mb |
(ftp)(http) |
TXT |
| GSE113795_GSM793372_N39_CD10.mapped.by.SAGE.genie.Best.Gene.txt.gz |
100.4 Kb |
(ftp)(http) |
TXT |
| GSE113795_GSM793372_N43_CD10.txt.gz |
11.2 Mb |
(ftp)(http) |
TXT |
| GSE113795_GSM793373_N40_CD10.mapped.by.SAGE.genie.Best.Gene.txt.gz |
99.9 Kb |
(ftp)(http) |
TXT |
| GSE113795_GSM793373_N48_CD10.txt.gz |
8.1 Mb |
(ftp)(http) |
TXT |
| GSE113795_GSM793374_N123_CD10.mapped.by.SAGE.genie.Best.Gene.txt.gz |
100.1 Kb |
(ftp)(http) |
TXT |
| GSE113795_GSM793374_N_CD10+_CD44-_N117_FC00302_s3_LIB002174.txt.gz |
21.5 Mb |
(ftp)(http) |
TXT |
| GSE113795_RAW.tar |
314.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...