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| Status |
Public on Feb 13, 2019 |
| Title |
DNA de novo (de)Methylation in the Kidney Contributes to Salt-Induced Hypertension [RNA-seq] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Numerous adult diseases involving tissues that consist primarily of non-dividing cells are associated with changes in DNA methylation. It suggests a role for de novo methylation or demethylation of DNA, which is catalyzed by DNA methyltransferase 3 (Dnmt3) and ten-eleven translocases (Tet). However, the contribution of DNA de novo (de)methylation to these diseases remains nearly completely unproven. Broad changes in DNA methylation occurred within days in the renal outer medulla of Dahl SS rats fed a high-salt diet, a classic model of hypertension. Intra-renal administration of anti-Dnmt3a/Tet3 GapmeR’s attenuated high salt-induced hypertension in SS rats. The high salt diet induced differential expression of 1,712 genes in the renal outer medulla. Remarkably, the differential expression of 76% of these genes were prevented by anti-Dnmt3a/Tet3 GapmeR’s. The genes differentially expressed in response to the GapmeR’s were involved in the regulation of metabolism and inflammation and were significantly enriched for genes showing differential methylation in response to the GapmeR’s. These data indicate DNA de novo (de)methylation in the kidney contributes to the development of hypertension in SS rats. The findings should help to shift the paradigm of DNA methylation research in diseases involving non-dividing cells from correlative analysis to functional and mechanistic studies.
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| Overall design |
Transcriptome analysis was performed in renal outer medulla tissues from individual rats (3 groups, n=3 per group).
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| Contributor(s) |
Liang M, Liu P |
| Citation(s) |
30354815 |
| Submission date |
May 10, 2018 |
| Last update date |
Feb 13, 2019 |
| Contact name |
Pengyuan Liu |
| E-mail(s) |
pliu@mcw.edu
|
| Phone |
4149552833
|
| Organization name |
Medical College of Wisconsin
|
| Department |
Physiology
|
| Street address |
8701 Watertown Plank Road
|
| City |
Milwaukee |
| State/province |
Wisconsin |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
|
| Samples (9)
|
| GSM3139725 |
MCW SS 0.4% salt_RNA-seq Replicate 1 [859] |
| GSM3139726 |
MCW SS 0.4% salt_RNA-seq Replicate 2 [860] |
| GSM3139727 |
MCW SS 0.4% salt_RNA-seq Replicate 3 [861] |
| GSM3139728 |
MCW SS HS_SCR_RNA-seq Replicate 1 [784] |
| GSM3139729 |
MCW SS HS_SCR_RNA-seq Replicate 2 [785] |
| GSM3139730 |
MCW SS HS_SCR_RNA-seq Replicate 3 [871] |
| GSM3139731 |
MCW SS HS_DT_RNA-seq Replicate 1 [781] |
| GSM3139732 |
MCW SS HS_DT_RNA-seq Replicate 2 [782] |
| GSM3139733 |
MCW SS HS_DT_RNA-seq Replicate 3 [862] |
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| This SubSeries is part of SuperSeries: |
| GSE114335 |
DNA de novo (de)Methylation in the Kidney Contributes to Salt-Induced Hypertension |
|
| Relations |
| BioProject |
PRJNA470983 |
| SRA |
SRP145418 |
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