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| Status |
Public on Aug 04, 2019 |
| Title |
Gene expression profile (GEP) of K562 cells expressing wtCALR, CALRins5 and CALRdel52 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In this work, we compared gene expression profile (GEP) of K562 cells transduced with the retroviral vector LCALRins5IDN or LCALRdel52IDN with K562 cells transduced with LwtCALRIDN
In order to unravel MPL-independent mechanisms underlying the effect of CALR mutations on MPN pathogenesis, we analysed the transcriptional changes induced by the CALRins5 or CALRdel52 overexpression in K562 cells, which lack MPL expression
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| Overall design |
In a set of three independent experiments, K562 cells were transduced with the LCALRwtIDN (CALR wild-type, CALRwt), LCALRdel52IDN (CALRdel52), LCALRins5IDN (CALRins5) retroviral vectors. After NGFR-based purification, the transduced cells were profiled by means of Affymetrix U219 Array
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| Contributor(s) |
Salati S, Genovese E, Prudente Z, Carretta C, Pennucci V, Ruberti S, Rontauroli S, Rossi C, Zini R, Bianchi E, Tagliafico E, Manfredini R |
| Citation(s) |
31332222 |
| Submission date |
May 14, 2018 |
| Last update date |
Aug 04, 2019 |
| Contact name |
Rossella Manfredini |
| E-mail(s) |
manfredini.rossella@unimore.it
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| Phone |
+390592058065
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| Organization name |
Centre for Regenerative Medicine
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| Department |
Life Sciences
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| Street address |
Via Gottardi 100
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| City |
Modena |
| ZIP/Postal code |
41100 |
| Country |
Italy |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA471305 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE114414_RAW.tar |
17.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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