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Series GSE114703 Query DataSets for GSE114703
Status Public on Jun 27, 2018
Title The Replication-initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Cell cycle progression in mammals is modulated by two ubiquitin ligase complexes, CRL4 and SCF, which facilitate degradation of chromatin substrates involved in the regulation of DNA replication. One member of the CRL4 complex, RepID (DCAF14/PHIP), is a WD40-containing protein that selectively binds and activates a group of replication origins. We found that RepID recruits the CRL4 complex to chromatin prior to DNA synthesis, thus playing a crucial architectural role in the proper licensing of chromosomes for replication. In the absence of RepID, cells rely on the alternative ubiquitin ligase, SKP2-containing SCF, to progress through the cell cycle. RepID depletion markedly increases cellular sensitivity to SKP2 inhibitors, which triggered massive genome re-replication. Both RepID and SKP2 interact with distinct, non-overlapping groups of replication origins, suggesting that selective interactions of replication origins with specific CRL components execute the DNA replication program and maintain genomic stability by preventing re-initiation of DNA replication.
Overall design Nascent strands were purified with the lambda exonuclease methods from U2OS RepID WT or HCT116 RepID KO cells. Chromatin from unsynchronized untreated cultures of U2OS RepID WT cells was subjected to ChIP-Seq with antibody directed against CUL4A, H3K4me3 and H3K9me3. Chromatin from unsynchronized untreated cultures of U2OS FLAG-RepID-FL overexpressed stable cells was subjected to ChIP-Seq with antibody directed against CDT2. Chromatin from unsynchronized untreated cultures of HCT116 RepID WT or RepID KO cells was subjected to ChIP-Seq with antibody directed against SKP2.
Contributor(s) Aladjem MI
Citation(s) 30018425
Submission date May 21, 2018
Last update date Feb 21, 2019
Contact name Christophe E Redon
Phone 240-760-7338
Organization name NIH
Street address 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (11)
GSM3147761 HCT116 RepID KO NS
GSM3147762 U2OS WT NS
GSM3147763 HCT116 WT SKP2 ChIP-seq
BioProject PRJNA472361
SRA SRP148578

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Supplementary file Size Download File type/resource
GSE114703_RAW.tar 5.8 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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