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| Status |
Public on Mar 13, 2019 |
| Title |
Investigation of the effect of 1,25(OH)2D3 on murine CD11+ dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression was compared between CD11c+ bone marrow derived dendritic cells (BMDC) was compared between cells conditioned in 20nM 1,25(OH)2D3 (Vitamin D) or vehicle. A second analysis compared gene expression in 1,25(OH)2D3 or vehicle CD11c+ BMDCs which were matured in presence of 0.1ug/ml LPS.
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| Overall design |
Murine bone marrow was harvested and cells grown for 9 days in presence of GM-CSF and either 20nM 1,25(OH)2D3 or vehicle. CD11c+ were isolated by FACS after which RNA was extracted. In some experiments the CD11c+ cells were stimulated with 0.1ug/ml LPS for an additional 18hours
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| Contributor(s) |
Mellanby RJ, Ivens AC |
| Citation(s) |
30984180 |
| Submission date |
May 22, 2018 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Alasdair Ivens |
| E-mail(s) |
al.ivens@ed.ac.uk
|
| Phone |
44 131 6513605
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| Organization name |
Centre for Immunity, Infection and Evolution
|
| Street address |
Kings Buildings
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3FL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA472589 |
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